ZFIN ID: ZDB-FIG-140602-17
Zannino et al., 2014 - prdm12b specifies the p1 progenitor domain and reveals a role for V1 interneurons in swim movements. Developmental Biology   390:247-60 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 6

Loss of prdm12b has a modest effect on hindbrain, but not spinal, motor neurons. (A–Q) Expression of Isl1/2 (A–D, K, L), olig2 (E–H), olig2:egfp (I, J), Isl1/2 together with hoxb1a:egfp (M, N) or Alcama (O–Q) in wild-type (A, C, E, F, I, K, M, O), PTB MO-injected (B, D, G, H, J, L, N, Q), or control MO-injected (P) embryos at 24 hpf (A–H) or 50 hpf (I–Q). Embryos are shown in lateral (A, B, I, J) or dorsal (C, D, K–Q) view with anterior to the left, or in cross section (E–H) with dorsal to the top. All scale bars are 35 μm, except I, J are 100 μm and E, H are 50 μm. RB – Rohon-Beard cells, MN – motor neurons, and (n)=cranial nerves.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
alcama WT control Long-pec facial nerve motor nucleus IHC
WT + MO1-prdm12b standard conditions Long-pec facial nerve motor nucleus IHC
EGFP um8Tg control Long-pec hindbrain IHC
um8Tg + MO1-prdm12b standard conditions Long-pec hindbrain IHC
vu12Tg control Long-pec spinal cord motor neuron IHC
vu12Tg + MO1-prdm12b standard conditions Long-pec spinal cord motor neuron IHC
olig2 WT control Prim-5 spinal cord central region ISH
WT + MO1-prdm12b standard conditions Prim-5 spinal cord central region ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-isl IHC WT control Prim-5 primary motor neuron
IHC Prim-5 Rohon-Beard neuron
IHC WT + MO1-prdm12b standard conditions Prim-5 primary motor neuron
IHC Prim-5 Rohon-Beard neuron
IHC um8Tg control Long-pec abducens motor nucleus
IHC Long-pec diencephalon
IHC Long-pec facial nerve motor nucleus
IHC Long-pec motor nucleus of vagal nerve
IHC Long-pec trigeminal motor nucleus
IHC um8Tg + MO1-prdm12b standard conditions Long-pec abducens motor nucleus
IHC Long-pec diencephalon
IHC Long-pec facial nerve motor nucleus
IHC Long-pec motor nucleus of vagal nerve
IHC Long-pec trigeminal motor nucleus
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-prdm12b standard conditions Prim-5 spinal cord has normal numbers of parts of type spinal cord motor neuron, normal
Prim-5 spinal cord motor neuron cell fate specification process quality, normal
Long-pec abducens motor nucleus has fewer parts of type abducens motor nucleus motor neuron, abnormal
um8Tg + MO1-prdm12b standard conditions Long-pec facial nerve motor nucleus motor neuron mislocalised, abnormal
vu12Tg + MO1-prdm12b standard conditions Long-pec spinal cord motor neuron cell fate specification process quality, normal
Acknowledgments:
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Reprinted from Developmental Biology, 390, Zannino, D.A., Downes, G.B., Sagerström, C.G., prdm12b specifies the p1 progenitor domain and reveals a role for V1 interneurons in swim movements, 247-60, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.