ZFIN ID: ZDB-FIG-140602-19
Zannino et al., 2014 - prdm12b specifies the p1 progenitor domain and reveals a role for V1 interneurons in swim movements. Developmental Biology   390:247-60 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-25 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-25 to Protruding-mouth

Fig. 8

Increased replication of OPCs in prdm12b deficient embryos. (A–I) olig2:egfp Expression in wild type (A–C), control MO-injected (D–F), or PTB MO-injected (G–I) embryos. (J) Quantification of embryos with reduced numbers of olig2:egfp+ cells. (K) Quantification of olig2:egfp+ cells per embryo at 72 hp. (L–O) Cross sections of wild type (L, M) and PTB MO-injected (N, O) Tg(olig2:egfp) embryos labeled with anti-BrdU. (P) Quantification of the number of olig2:egfp+/BrdU+ cells per section. Scale bars are 35 μm (A–I) or 17 μm (L, M). The neural tube is outlined with dotted line in L, M. Arrows indicate olig2:egfp/BrdU double positive cells.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP vu12Tg control Prim-25 rhombomere 5 glioblast (sensu Vertebrata) IHC
Prim-25 rhombomere 6 glioblast (sensu Vertebrata) IHC
Long-pec hindbrain glioblast (sensu Vertebrata) IHC
Protruding-mouth hindbrain oligodendrocyte IHC
vu12Tg standard conditions Prim-25 rhombomere 5 glioblast (sensu Vertebrata) IHC
Prim-25 rhombomere 6 glioblast (sensu Vertebrata) IHC
Long-pec hindbrain glioblast (sensu Vertebrata) IHC
Protruding-mouth hindbrain oligodendrocyte IHC
vu12Tg + MO1-prdm12b standard conditions Prim-25 rhombomere 5 glioblast (sensu Vertebrata) IHC
Prim-25 rhombomere 6 glioblast (sensu Vertebrata) IHC
Long-pec hindbrain glioblast (sensu Vertebrata) IHC
Protruding-mouth hindbrain oligodendrocyte IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
vu12Tg + MO1-prdm12b standard conditions Prim-25 hindbrain has fewer parts of type glioblast (sensu Vertebrata), abnormal
Long-pec hindbrain has fewer parts of type glioblast (sensu Vertebrata), abnormal
Long-pec oligodendrocyte progenitor proliferation occurrence, normal
Protruding-mouth hindbrain has normal numbers of parts of type oligodendrocyte, normal
Protruding-mouth oligodendrocyte progenitor proliferation increased occurrence, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 390, Zannino, D.A., Downes, G.B., Sagerström, C.G., prdm12b specifies the p1 progenitor domain and reveals a role for V1 interneurons in swim movements, 247-60, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.