ZFIN ID: ZDB-FIG-140602-20
Zannino et al., 2014 - prdm12b specifies the p1 progenitor domain and reveals a role for V1 interneurons in swim movements. Developmental Biology   390:247-60 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 4

Fig. 9

Fish lacking prdm12b function display an abnormal touch response. (A, B) Kinematic traces for 10 control MO-injected (A) and 10 PTB MO-injected (B) 4dpf fish from one of three separate experiments. Zero degrees on the y-axis indicate a straight body while positive and negative angles represent body bends in opposite directions. (C, D) Anti-3A10 labeling of Mauthner neurons in wild type (C) and PTB MO-injected (D) embryos in dorsal view. (E) Quantification of body bends with amplitude similar to the C-bend (defined as exceeding 110┬░). (F) Quantification of escape response duration in milliseconds. (G, H) Overlay of frames taken every 20 ms from a high-speed recording of a touch response in control MO-injected (G) and PTB MO-injected (H) fish. (I) Quantification of distance traveled during the escape response. (J) Quantification of time interval between the first and second bend (bend 1), second and third bend (bend 2), third and fourth bend (bend 3) during the escape response.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-3A10 IHC WT standard conditions Day 4 Mauthner neuron
IHC WT + MO1-prdm12b standard conditions Day 4 Mauthner neuron
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-prdm12b standard conditions Day 4 Mauthner neuron morphology, normal
Day 4 thigmotaxis increased duration, abnormal
Day 4 thigmotaxis increased magnitude, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 390, Zannino, D.A., Downes, G.B., Sagerstr├Âm, C.G., prdm12b specifies the p1 progenitor domain and reveals a role for V1 interneurons in swim movements, 247-60, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.