|ZFIN ID: ZDB-FIG-140602-20|
Fish lacking prdm12b function display an abnormal touch response. (A, B) Kinematic traces for 10 control MO-injected (A) and 10 PTB MO-injected (B) 4dpf fish from one of three separate experiments. Zero degrees on the y-axis indicate a straight body while positive and negative angles represent body bends in opposite directions. (C, D) Anti-3A10 labeling of Mauthner neurons in wild type (C) and PTB MO-injected (D) embryos in dorsal view. (E) Quantification of body bends with amplitude similar to the C-bend (defined as exceeding 110°). (F) Quantification of escape response duration in milliseconds. (G, H) Overlay of frames taken every 20 ms from a high-speed recording of a touch response in control MO-injected (G) and PTB MO-injected (H) fish. (I) Quantification of distance traveled during the escape response. (J) Quantification of time interval between the first and second bend (bend 1), second and third bend (bend 2), third and fourth bend (bend 3) during the escape response.
|Ab-3A10||IHC||WT||standard conditions||Day 4||Mauthner neuron|
|IHC||WT + MO1-prdm12b||standard conditions||Day 4||Mauthner neuron|
|WT + MO1-prdm12b||standard conditions||Day 4||Mauthner neuron morphology, normal|
|Day 4||thigmotaxis increased duration, abnormal|
|Day 4||thigmotaxis increased magnitude, abnormal|
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Reprinted from Developmental Biology, 390, Zannino, D.A., Downes, G.B., Sagerström, C.G., prdm12b specifies the p1 progenitor domain and reveals a role for V1 interneurons in swim movements, 247-60, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.