ZFIN ID: ZDB-FIG-140304-5
Su et al., 2014 - Cerebellar development in the absence of Gbx function in zebrafish. Developmental Biology   386(1):181-90 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: 26+ somites to Day 6
PHENOTYPE:
Fish:
Observed In:
Stage: Day 6

Fig. 1

Gbx1 and Gbx2 function redundantly in cerebellum development. (A) Schematic of nonsense mutations identified in zebrafish gbx1 and gbx2 by TILLING. Both mutations are expected to prevent DNA binding by truncating the homeodomain. (B–I) Dorsal views at 22 hpf (B–E) or 6 dpf (F–I), anterior to the left. Genotypes are shown at the top. otx2 (blue), eng2b (red), and egr2b (blue) are expressed in midbrain, midbrain–hindbrain boundary (MHB), and rhombmere 3/5, respectively, in wildtype (WT), single and double mutants as shown. Tg(ptf1a:EGFP) (green) marks Purkinje neuron progenitors, Tg(olig2:DsRed2) (red) marks projection neurons, and anti-Vglut1/Slc17a7 (blue) marks cerebellar granule neuron axons. In gbx1fh271;gbx2fh253 double mutants the midbrain is expanded at the expense of r1 and no cerebellum forms.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 gbx1fh271/fh271; gbx2fh253/fh253; jh1Tg; vu19Tg standard conditions Day 6 neuron neuron projection IHC
gbx1fh271/fh271; jh1Tg; vu19Tg standard conditions Day 6 neuron neuron projection IHC
gbx2fh253/fh253; jh1Tg; vu19Tg standard conditions Day 6 neuron neuron projection IHC
jh1Tg; vu19Tg standard conditions Day 6 neuron neuron projection IHC
EGFP gbx1fh271/fh271; gbx2fh253/fh253; jh1Tg; vu19Tg standard conditions Day 6 Purkinje cell IHC
gbx1fh271/fh271; jh1Tg; vu19Tg standard conditions Day 6 Purkinje cell IHC
gbx2fh253/fh253; jh1Tg; vu19Tg standard conditions Day 6 Purkinje cell IHC
jh1Tg; vu19Tg standard conditions Day 6 Purkinje cell IHC
egr2b WT standard conditions 26+ somites rhombomere 3 ISH
26+ somites rhombomere 5 ISH
gbx1fh271/fh271 standard conditions 26+ somites rhombomere 3 ISH
26+ somites rhombomere 5 ISH
gbx1fh271/fh271; gbx2fh253/fh253 standard conditions 26+ somites rhombomere 3 ISH
26+ somites rhombomere 5 ISH
gbx2fh253/fh253 standard conditions 26+ somites rhombomere 3 ISH
26+ somites rhombomere 5 ISH
en2b WT standard conditions 26+ somites midbrain posterior region ISH
26+ somites rhombomere 1 ISH
gbx1fh271/fh271 standard conditions 26+ somites midbrain posterior region ISH
26+ somites rhombomere 1 ISH
gbx1fh271/fh271; gbx2fh253/fh253 standard conditions 26+ somites midbrain posterior region ISH
gbx2fh253/fh253 standard conditions 26+ somites midbrain posterior region ISH
26+ somites rhombomere 1 ISH
otx2b WT standard conditions 26+ somites midbrain ISH
gbx1fh271/fh271 standard conditions 26+ somites midbrain ISH
gbx1fh271/fh271; gbx2fh253/fh253 standard conditions 26+ somites midbrain ISH
gbx2fh253/fh253 standard conditions 26+ somites midbrain ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-slc17a7 IHC gbx1fh271/fh271; gbx2fh253/fh253; jh1Tg; vu19Tg standard conditions Day 6 cerebellar granule cell axon
IHC gbx1fh271/fh271; jh1Tg; vu19Tg standard conditions Day 6 cerebellar granule cell axon
IHC gbx2fh253/fh253; jh1Tg; vu19Tg standard conditions Day 6 cerebellar granule cell axon
IHC jh1Tg; vu19Tg standard conditions Day 6 Not Detected cerebellar granule cell axon
Phenotype Details
Fish Conditions Stage Phenotype
gbx1fh271/fh271; gbx2fh253/fh253; jh1Tg; vu19Tg standard conditions Day 6 cerebellum aplastic, abnormal
Day 6 cerebellum development process quality, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 386(1), Su, C.Y., Kemp, H.A., and Moens, C.B., Cerebellar development in the absence of Gbx function in zebrafish, 181-90, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.