FIGURE

Fig. 3

ID

ZDB-FIG-130211-40

Publication

Ma et al., 2012 - Foxn1 maintains thymic epithelial cells to support T-cell development via mcm2 in zebrafish

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Fig. 3

T-cell development is impaired in zebrafish cdca7 and mcm2 morphants. (A) The protein expression of Cdca7 and Mcm2 in the zebrafish cdca7 and mcm2 morphants detected by using Western blot. (B) The expression of T-cell markers (rag1, il7r, and ikaros) in zebrafish cdca7 and mcm2 morphants by qRT-PCR (mean ± SD, Student’s t test, **P < 0.01, n = 3). (C) The expression of rag1, il7r, ikaros, and foxn1 in zebrafish cdca7 and mcm2 morphants by WISH. Anterior to the left and dorsal to the up; circles mark the thymus.

Expression Data

Genes:	foxn1 ikzf1 il7r rag1
Fish:	WT WT + MO1-cdca7a WT + MO1-mcm2
Knockdown Reagents:	MO1-cdca7a MO1-mcm2
Anatomical Terms:	lymphoid progenitor cell T cell thymus thymus epithelial cell
Stage:	Day 4

Expression Detail

Gene	Fish	Conditions	Stage	Anatomy	Assay
foxn1	WT	standard conditions	Day 4	thymus	ISH
foxn1	WT	standard conditions	Day 4	thymus epithelial cell	ISH
foxn1	WT + MO1-cdca7a	standard conditions	Day 4	thymus	ISH
foxn1	WT + MO1-cdca7a	standard conditions	Day 4	thymus epithelial cell	ISH
foxn1	WT + MO1-mcm2	standard conditions	Day 4	thymus	ISH
foxn1	WT + MO1-mcm2	standard conditions	Day 4	thymus epithelial cell	ISH
ikzf1	WT	standard conditions	Day 4	lymphoid progenitor cell	ISH
ikzf1	WT	standard conditions	Day 4	T cell	ISH
ikzf1	WT	standard conditions	Day 4	thymus	ISH
ikzf1	WT + MO1-cdca7a	standard conditions	Day 4	lymphoid progenitor cell	ISH

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA