ZFIN Figure: Das et al., 2012, Fig. 8

ZFIN ID: ZDB-FIG-120522-22

ZFIN ID: ZDB-FIG-120522-22

Das et al., 2012 - Bmps and id2a act upstream of twist1 to restrict ectomesenchyme potential of the cranial neural crest. PLoS Genetics 8(5):e1002710 Full text @ PLoS Genet.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	aox5 dct dlx2a fli1 foxd3 KillerRed sox10
Fish:	el159Tg; el15Tg el159Tg; el15Tg; el49Tg
Anatomical Terms:	glial cell melanocyte otic vesicle pharyngeal arch pharyngeal arch 1 pharyngeal arch 2 pharyngeal arch 3 pharyngeal arch 4 xanthoblast
Stage Range:	14-19 somites to Long-pec

PHENOTYPE:

Fish:	el159Tg; el15Tg; el49Tg
Observed In:	splanchnocranium
Stage:	Day 5

Fig. 8

Misexpression of Bmp4 in migrating CNCCs inhibits ectomesenchyme formation.

(A–D) Whole mount in situs at 18 hpf show ectopic expression of sox10 in the arches (numbered) of sox10:Gal4VP16; UAS:Bmp4; UAS:mKR embryos (n = 8/8) compared to sox10:Gal4VP16; UAS:mKR controls (n = 0/8) and reductions of dlx2a in sox10:Gal4VP16; UAS:Bmp4; UAS:mKR embryos (n = 4/4) compared to sox10:Gal4VP16; UAS:mKR controls (n = 0/4). Arrows indicate the second arch and white arrowheads the developing ear. (E–H) Double fluorescent in situs for mKR (red) and fli1a or dlx2a (green) at 24 hpf show reduction of fli1a arch expression in sox10:Gal4VP16; UAS:Bmp4; UAS:mKR embryos (n = 5/5) compared to sox10:Gal4VP16; UAS:mKR controls (n = 0/9) and reduction of dlx2a arch expression in sox10:Gal4VP16; UAS:Bmp4; UAS:mKR embryos (n = 4/4) compared to sox10:Gal4VP16; UAS:mKR controls (n = 0/3). The mKR transgene was included as a lineage tracer for CNCC-derived cells that migrated into the arches. (I,J) Skeletal staining at 5 dpf shows severe loss of craniofacial skeleton in sox10:Gal4VP16; UAS:Bmp4; UAS:mKR embryos (n = 7/7) compared to sox10:Gal4VP16; UAS:mKR controls (n = 0/9). (K,L) In situs for foxd3 at 48 hpf reveal largely normal patterns of glia in sox10:Gal4VP16; UAS:Bmp4; UAS:mKR embryos (n = 8) and sox10:Gal4VP16; UAS:mKR controls (n = 11). (M–P) In situs at 28 hpf show normal dct-positive melanophore precursors in sox10:Gal4VP16; UAS:Bmp4; UAS:mKR embryos (n = 12) and sox10:Gal4VP16; UAS:mKR controls (n = 15) and normal xdh-positive xanthophore precursors in sox10:Gal4VP16; UAS:Bmp4; UAS:mKR embryos (n = 10) and sox10:Gal4VP16; UAS:mKR controls (n = 10). Scale bars = 50 μm.

Gene Expression Details

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay
aox5	el159Tg; el15Tg	standard conditions	Prim-5		xanthoblast	ISH
	el159Tg; el15Tg; el49Tg	standard conditions	Prim-5		xanthoblast	ISH
dct	el159Tg; el15Tg	standard conditions	Prim-5		melanocyte	ISH
	el159Tg; el15Tg; el49Tg	standard conditions	Prim-5		melanocyte	ISH
dlx2a	el159Tg; el15Tg	standard conditions	14-19 somites		pharyngeal arch 1	ISH
			14-19 somites		pharyngeal arch 2	ISH
			14-19 somites		pharyngeal arch 3	ISH
			Prim-5		pharyngeal arch	ISH
			Prim-5		pharyngeal arch 1	ISH
			Prim-5		pharyngeal arch 2	ISH
			Prim-5		pharyngeal arch 3	ISH
			Prim-5		pharyngeal arch 4	ISH
	el159Tg; el15Tg; el49Tg	standard conditions	14-19 somites		pharyngeal arch 1	ISH
			14-19 somites		pharyngeal arch 2	ISH
			14-19 somites		pharyngeal arch 3	ISH
			Prim-5	Not Detected	pharyngeal arch	ISH
			Prim-5	Not Detected	pharyngeal arch 1	ISH
			Prim-5	Not Detected	pharyngeal arch 2	ISH
			Prim-5	Not Detected	pharyngeal arch 3	ISH
fli1	el159Tg; el15Tg	standard conditions	Prim-5		pharyngeal arch	ISH
			Prim-5		pharyngeal arch 1	ISH
			Prim-5		pharyngeal arch 2	ISH
			Prim-5		pharyngeal arch 3	ISH
	el159Tg; el15Tg; el49Tg	standard conditions	Prim-5		pharyngeal arch 1	ISH
			Prim-5		pharyngeal arch 2	ISH
			Prim-5		pharyngeal arch 3	ISH
foxd3	el159Tg; el15Tg	standard conditions	Long-pec		glial cell	ISH
	el159Tg; el15Tg; el49Tg	standard conditions	Long-pec		glial cell	ISH
KillerRed	el159Tg; el15Tg	standard conditions	Prim-5		pharyngeal arch	ISH
			Prim-5		pharyngeal arch 1	ISH
			Prim-5		pharyngeal arch 2	ISH
			Prim-5		pharyngeal arch 3	ISH
			Prim-5		pharyngeal arch 4	ISH
	el159Tg; el15Tg; el49Tg	standard conditions	Prim-5		pharyngeal arch	ISH
			Prim-5		pharyngeal arch 1	ISH
			Prim-5		pharyngeal arch 2	ISH
			Prim-5		pharyngeal arch 3	ISH
sox10	el159Tg; el15Tg	standard conditions	14-19 somites		otic vesicle	ISH
			14-19 somites	Not Detected	pharyngeal arch	ISH
	el159Tg; el15Tg; el49Tg	standard conditions	14-19 somites		otic vesicle	ISH
			14-19 somites		pharyngeal arch	ISH
			14-19 somites		pharyngeal arch 1	ISH
			14-19 somites		pharyngeal arch 2	ISH
			14-19 somites		pharyngeal arch 3	ISH

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
el159Tg; el15Tg; el49Tg	standard conditions	Day 5	splanchnocranium hypoplastic, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.