FIGURE

Fig. S1

ID
ZDB-FIG-120522-25
Publication
Das et al., 2012 - Bmps and id2a act upstream of twist1 to restrict ectomesenchyme potential of the cranial neural crest
Other Figures
All Figure Page
Back to All Figure Page
Fig. S1

Twist1a and Twist1b function redundantly to specify ectomesenchyme. (A–C) In situs at 18 hpf show sox10 expression in un-injected, twist1a-MO, and twist1b-MO embryos. A few ectopic sox10-positive cells are seen in the second arches (arrows) of twist1a-MO and twist1b-MO embryos. White arrowheads denote the developing ear. (D–G) Confocal projections of fli1a:GFP; sox10:dsRed doubly transgenic embryos at 28 hpf show normal fli1a:GFP expression in un-injected control, twist1a-MO, and twist1b-MO embryos and loss of fli1a:GFP arch expression in twist1a/1b-MO embryos. Arrows indicate fli1a:GFP vascular expression which is unaffected in twist1a/1b-MO embryos. (H–L) Skeletal staining shows malformed mandibular and hyoid skeletons in twist1a-MO and twist1b-MO embryos compared to un-injected controls. In addition, co-injection of a Twist1b mRNA not targeted by the MOs partially rescued the head skeleton of twist1a/1b-MO embryos (n = 24/24), whereas co-injection of a control kikGR mRNA never rescued (n = 0/11). Scale bars = 50 μm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.