FIGURE

Fig. 5

ID
ZDB-FIG-110624-10
Publication
Ikenaga et al., 2011 - Formation of the spinal network in zebrafish determined by domain-specific pax genes
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Fig. 5

RFP expression in the central nervous system. A,B: Confocal images displaying RFP+ cells in the hindbrain. A is a dorsal view of the hindbrain, and B is a higher magnification of the ventral region. Note the rostral end of the hindbrain lacks RFP+ cells (A). In the ventral region (B), axon-like structures were detected crossing the midline (arrowhead). sc, Spinal cord. C,D: RFP+ cells (red) in the hindbrain compared with the islet-1 GFP-expressing motor neurons (green). C is a stack of confocal images. D displays a single confocal plane near the Xth (X) motor nuclei. Note that two populations (red and green) do not overlap. E–H: A longitudinal series of the hindbrain sections is shown from the level of IIIrd, IVth (E), Vth (F), VIIth (G), and Xth (H) motor nucleus. RFP+ cells are red, and islet-1 expressing motor neurons are green. I,J: RFP+ cells (red) in the hindbrain were compared with the reticular neurons stained by the tracing dye, dextran fluorescein (green). I is a stack of confocal images and J is a single plane at the level of the Mauthner cell (M). S4 through S7 indicates segment number. Magenta-green images are provided in Supporting Information Figure 3. Scale bars = 50 μm in A–D,I; 100 μm in H (applies to E–H); 20 μm in J.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Comp. Neurol.