FIGURE

Fig. 4

ID

ZDB-FIG-101108-4

Publication

Hellman et al., 2010 - The zebrafish foxj1a transcription factor regulates cilia function in response to injury and epithelial stretch

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Fig. 4

foxj1a target genes are up-regulated in response to tubule obstruction. Control expression of foxj1a target genes efhc1 (A), tektin-1 (D), and dnahc9 (G) at 56 hpf is up-regulated by distal pronephric obstruction (B, E, and H). foxj1a knockdown prevented up-regulation in response to tubule obstruction (C, F, and I). Quantitative real-time RT-PCR (J) confirmed up-regulation of foxj1a target gene expression in obstructed tubules (dnahc9 13.3-fold; efch1 3.4-fold, and tektin1 2.6-fold) and inhibition of induction in foxj1a-deficient obstructed tubules. Error bars in J represent range of minimum and maximum fold foxj1a induction relative to control embryos (comparative threshold cycle method) (59).

Expression Data

Genes:	dnah9 efhc1 tekt1
Fish:	WT WT + MO5-foxj1a + MO6-foxj1a
Condition:	physical alteration: anatomical structure
Knockdown Reagents:	MO5-foxj1a MO6-foxj1a
Anatomical Terms:	pronephric tubule multi-ciliated epithelial cell pronephric tubule single ciliated epithelial cell whole organism
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + MO5-foxj1a + MO6-foxj1a
Condition:	physical alteration: anatomical structure
Knockdown Reagents:	MO5-foxj1a MO6-foxj1a
Observed In:	renal tubule development response to wounding
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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