FIGURE

Fig. 3

ID
ZDB-FIG-101108-3
Publication
Hellman et al., 2010 - The zebrafish foxj1a transcription factor regulates cilia function in response to injury and epithelial stretch
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Fig. 3

foxj1a is required for cilia motility. (A) Control scorpion-eGFP-labeled spinal canal cilia in 24-hpf embryos beat rapidly as seen in high-speed line scanning over time (16.9 beats/s in this example; total time, 768 ms. (Scale bar, 100 ms.) (A, Inset) Single cilia imaged in slow-frame scan appears as squiggled line pattern; red line in Inset indicates line acquired in high-speed line scan. (B) High-speed line scan of foxj1a 24-hpf morphant spinal cord cilia shows no motility (total time, 768 ms). (Scale bar, 100 ms.) (B, Inset) Single cilia imaged in slow frame scan shows no movement; red line in inset indicates line acquired in high-speed line scan. (C) Control Kupffer′s vesicle cilia (12-somite stage) beat rapidly, as seen in high-speed line scanning over time (27 beats/s in this example; total time, 768 ms). (Scale bar, 100 ms.) (C, Inset) Single cilia at edge of Kupffer′s vesicle imaged in slow frame scan appears as squiggled line pattern; red line in Inset indicates line acquired in high-speed line scan. (D) High-speed line scan of foxj1a morphant Kupffer′s cilia (12-somite stage) shows no motility (total time, 768 ms). (Scale bar, 100 ms.) (D, Inset) Single cilia imaged in slow frame scan shows no movement; red line in inset indicates line acquired in high-speed line scan.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 10-13 somites to Prim-5

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA