FIGURE

Fig. 6

ID
ZDB-FIG-100628-27
Publication
Kani et al., 2010 - Proneural gene-linked neurogenesis in zebrafish cerebellum
Other Figures
All Figure Page
Back to All Figure Page
Fig. 6

Differentiation and migration of olig2+ eurydendroid cells. (A–F) Expression of olig2 (B, D, F) and olig2:EGFP (A, C, E) in the cerebellum at 2, 3, and 5 dpf. Dorsal views with rostral to the left. (G–J) Expression of olig2:EGFP and vglut2:DsRed at 3 (G, H) and 5 (I, J) dpf. EGFP and DsRed signals in Tg(olig2:EGFP); Tg(vglut2a:DsRed) were detected with anti-EGFP (green) and anti-DsRed (magenta) antibodies, respectively. (H, J) Sagittal sections at the levels indicated in G and I, respectively. The olig2:EGFP+ cells in the VZ were negative for vglut2a:DsRed (arrowheads), but in the GCL and PCL domains, most olig2:EGFP+ cells were positive for vglut2a:DsRed (arrows). (K–O) Expression of olig2:EGFP and ptf1a:Gal4VP16; UAS:NfsB-mCherry (ptf1a:G4-U:mCherry) at 2 dpf (K, L), 5 dpf (M, N), and the adult cerebellum (O). (K, M) Dorsal views; (L) high-magnification view of the box in (K); (N) sagittal section at the level indicated in (M); (O) sagittal section. Note that most olig2:EGFP+ cells were also positive for ptf1a:G4-UAS:mCherry at 2 dpf (arrows), but many of them became negative after differentiation at 5 dpf. olig2:EGFP+ ptf1a:G4-U:mCherry+ cells were also detected in the VZ at 5 dpf and adult stage (arrowheads). (P-S) Expression of olig2:EGFP and atoh1a:dTomato at 2 (P, Q) and 5 (R, S) dpf. (Q, S) High-magnification views of the boxes in (P, R). The olig2:EGFP+ cells located in the lateral domains were positive for atoh1a:dTomato (arrows). (T) Schematic representation of development of the olig2+ eurydendroid cells. The abbreviations are defined in Fig. 1. Scale bars: 50 μm (H, S), 100 μm (L), 200 μm (A, B, G, K, O, P).

Expression Data
Genes:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Day 5

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 343(1-2), Kani, S., Bae, Y.K., Shimizu, T., Tanabe, K., Satou, C., Parsons, M.J., Scott, E., Higashijima, S.I., and Hibi, M., Proneural gene-linked neurogenesis in zebrafish cerebellum, 1-17, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.