FIGURE

Fig. 3

ID
ZDB-FIG-100628-24
Publication
Kani et al., 2010 - Proneural gene-linked neurogenesis in zebrafish cerebellum
Other Figures
All Figure Page
Back to All Figure Page
Fig. 3

Behavior of granule cells. (A–D) Expression of neurod at 3 (A, B) and 5 (C, D) dpf. Dorsal views (A, B, C, D) and sagittal sections at the levels indicated in B and D (Ba, Da, Db). Neurod+ mature granule cells in the granule cell layer are marked by arrows. (E, F) Expression of atoh1a:EGFP (green) and Neurod (magenta) at 3 (E) and 5 dpf (F, G). Sagittal sections at the levels comparable to Ba (for E), Da (for F), and Db (for G). atoh1a:EGFP+ Neurod+ cells are marked by arrows. (H–K) BrdU incorporation at 3 dpf. Tg(atoh1a:EGFP) (H) or wild-type larvae (I–K) were labeled with bromodeoxyuridine (BrdU) for 1 h, and proliferating cells were analyzed by immunostaining with anti-BrdU (magenta in H and K; brown in I and J) antibody, and anti-EGFP (green, H), anti-Neurod (green, K) antibodies, atoh1b (I) or atoh1c (J) riboprobes sagittal sections. The larvae were stained with anti-BrdU (magenta) and anti-Neurod (green) antibodies. Cells that were positive both for BrdU signals and proneural gene expression are marked by arrows. (L, M) Birthdate analysis. Larvae were labeled with BrdU at 5 dpf and fixed 1 h after the labeling (L) or at 7 dpf (two days after the labeling, M). Dorsal view (L) and sagittal sections at the mediolateral level (La and M). BrdU+ Neurod+ cells are marked by arrows. (N) Schematic representation of granule cell development during the larval period. TeO, tectum opticum; GCL, granule cell layer; other abbreviations are described in Fig. 1. Scale bars: 50 μm (Ba, E, H), 100 μm (L), 200 μm (A, B).

Expression Data
Genes:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 343(1-2), Kani, S., Bae, Y.K., Shimizu, T., Tanabe, K., Satou, C., Parsons, M.J., Scott, E., Higashijima, S.I., and Hibi, M., Proneural gene-linked neurogenesis in zebrafish cerebellum, 1-17, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.