FIGURE

Fig. 4

ID
ZDB-FIG-090717-25
Publication
Winata et al., 2009 - Development of zebrafish swimbladder: the requirement of Hedgehog signaling in specification and organization of the three tissue layers
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Fig. 4

Characterization of the role of Hh pathway in swimbladder development. (A–D) EGFP expression in Et(krt4:EGFP)sq33-2 in wild type (A), syut4(B), Ihha morphants (C), and smob641 (D) backgrounds. Panel (A) shows normal swimbladder epithelium in wild type control and the swimbladder is hypoplastic in syut4(B), reduced in Ihha morphants (C), and absent in smob641 (D, white arrowheads). (E–H) Expression of hb9 was normal in wild type control (E), almost absent in syut4 (F) reduced in Ihha morphants (G), and absent in smob641 (H) at 72 hpf. (I–L) Mesenchymal fgf10 expression is normal in wild type (I), reduced and disorganized in syut4 (J), reduced and disorganized in Ihha morphants (K), and absent in smob641 (L). (M–P) Smooth muscle is absent in all experimental larvae as indicated by the absence of acta2 expression. (Q–T) Mesothelial anxa5 expression was well-organized in wild type controls (Q), disorganized and presented as two lateral stripes of cells (arrowhead) in syut4 mutants (R), organized but severely reduced in Ihha morphants (S), and significantly reduced and presented as scattered cell clusters in smob641 mutants (T). The epithelial expression in Ihha morphants is denoted by white arrowhead. (U–X) cross sections of 4 dpf control (U), syut4 (V), Ihha morphants (W) and smob641 (X) larvae stained by hematoxylin–eosin. Abbreviations: ba, branchial arches; e, epithelium; g, gut; m, mesenchyme; nt, neural tube; n, notochord; o, outer mesothelium; sb, swimbladder. Scale bars: 250 mm.

Expression Data
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 5

Phenotype Detail
Acknowledgments
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Reprinted from Developmental Biology, 331(2), Winata, C.L., Korzh, S., Kondrychyn, I., Zheng, W., Korzh, V., and Gong, Z., Development of zebrafish swimbladder: the requirement of Hedgehog signaling in specification and organization of the three tissue layers, 222-236, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.