PUBLICATION

Development of zebrafish swimbladder: the requirement of Hedgehog signaling in specification and organization of the three tissue layers

Authors

Winata, C.L., Korzh, S., Kondrychyn, I., Zheng, W., Korzh, V., and Gong, Z.

ID

ZDB-PUB-090511-18

Date

2009

Source

Developmental Biology 331(2): 222-236 (Journal)

Registered Authors

Gong, Zhiyuan, Kondrychyn, Igor, Korzh, Svitlana, Korzh, Vladimir, Winata, Cecilia Lanny

Keywords

zebrafish, swimbladder, Hedgehog, fgf10, enhancer trap, lung

MeSH Terms

Animals
Air Sacs/embryology*
Air Sacs/physiology
Zebrafish Proteins/genetics
Zebrafish Proteins/physiology*
Embryo, Nonmammalian
Antigens, Differentiation/metabolism
Hedgehog Proteins/genetics
Hedgehog Proteins/physiology*
Mutation
Zebrafish/embryology
Zebrafish/physiology*
Signal Transduction
Body Patterning/physiology

(all 14)

PubMed

19422819 Full text @ Dev. Biol.

Abstract

The swimbladder is a hydrostatic organ in fish postulated as a homolog of the tetrapod lung. While lung development has been well studied, the molecular mechanism of swimbladder development is essentially uncharacterized. In the present study, swimbladder development in zebrafish was analyzed by using several molecular markers: hb9 (epithelium), fgf10a and acta2 (mesenchyme), and anxa5 (mesothelium), as well as in vivo through enhancer trap transgenic lines Et(krt4:EGFP)(sq33-2) and Et(krt4:EGFP)(sqet3) that showed strong EGFP expression in the swimbladder epithelium and outer mesothelium respectively. We defined three phases of swimbladder development: epithelial budding between 36-48 hpf, growth with the formation of two additional mesodermal layers up to 4.5 dpf, and inflation of posterior and anterior chambers at 4.5 and 21 dpf respectively. Similar to those in early lung development, conserved expression of Hedgehog (Hh) genes, shha and ihha, in the epithelia, and Hh receptor genes, ptc1 and ptc2, as well as fgf10a in mesencyme was observed. By analyzing several mutants affecting Hh signaling and Ihha morphants, we demonstrated an essential role of Hh signaling in swimbladder development. Furthermore, time-specific Hh inhibition by cyclopamine revealed different requirements of Hh signaling in the formation and organization of all three tissue layers of swimbladder.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
acta2	AB	standard conditions	Protruding-mouth		swim bladder smooth muscle cell	ISH	Fig. 1
acta2	AB	standard conditions	Protruding-mouth		swim bladder smooth muscle cell	ISH	Fig. 4
acta2	AB	standard conditions	Pec-fin		swim bladder smooth muscle cell	ISH	Fig. 1
acta2	AB + MO1-ihha	standard conditions	Protruding-mouth	Not Detected	swim bladder smooth muscle cell	ISH	Fig. 4
acta2	Df(Chr07)t4/t4	standard conditions	Protruding-mouth	Not Detected	swim bladder smooth muscle cell	ISH	Fig. 4
acta2	smo^b641/b641	standard conditions	Protruding-mouth	Not Detected	swim bladder smooth muscle cell	ISH	Fig. 4
anxa5b	AB	standard conditions	Protruding-mouth		swim bladder epithelium	ISH	Fig. 1
anxa5b	AB	standard conditions	Pec-fin		swim bladder epithelium	ISH	Fig. 1
anxa5b	AB	standard conditions	Protruding-mouth		swim bladder mesothelial cell	ISH	Fig. 1
anxa5b	AB	standard conditions	Pec-fin		swim bladder mesothelial cell	ISH	Fig. 1

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
AB + MO1-ihha	standard conditions	Long-pec	swim bladder bud hypoplastic, abnormal	Fig. S3
AB + MO1-ihha	standard conditions	Long-pec	swim bladder morphogenesis disrupted, abnormal	Fig. S3
AB + MO1-ihha	standard conditions	Pec-fin	smoothened signaling pathway disrupted, abnormal	Fig. S7
AB + MO1-ihha	standard conditions	Pec-fin	swim bladder formation disrupted, abnormal	Fig. S7
AB + MO1-ihha	standard conditions	Pec-fin	swim bladder hypoplastic, abnormal	Fig. S7
AB + MO1-ihha	standard conditions	Protruding-mouth	swim bladder epithelium decreased size, abnormal	Fig. 4
AB + MO1-ihha	standard conditions	Protruding-mouth	swim bladder hypoplastic, abnormal	Fig. 4
AB + MO1-ihha	standard conditions	Protruding-mouth	swim bladder hypoplastic, abnormal	Fig. S3
AB + MO1-ihha	standard conditions	Protruding-mouth	swim bladder mesenchyme decreased size, abnormal	Fig. 4
AB + MO1-ihha	standard conditions	Protruding-mouth	swim bladder mesenchyme disorganized, abnormal	Fig. 4

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
b641		Point Mutation	smo
sqet3Et	Et(krt4:EGFP)	Transgenic Insertion
sqet332Et	Et(krt4:EGFP)	Transgenic Insertion
t4		Deficiency	shha

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
ihha	MO1-ihha	MRPHLNO
tp53	MO4-tp53	MRPHLNO

Fish

Fish
AB
AB + MO1-ihha
AB + MO1-ihha + MO4-tp53
Df(Chr07)t4/t4
Df(Chr07)t4/t4; sqet332Et
smo^b641/b641
smo^b641/b641; sqet332Et
sqet3Et
sqet332Et
sqet332Et + MO1-ihha

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP

Mapping

Entity Type	Entity Symbol	Location
Feature	sqet332Et	Chr: 6 Details
GENE	zgc:112416	Chr: 6 Details

Winata et al., 2009 ZDB-PUB-090511-18 PMID:19422819

Development of zebrafish swimbladder: the requirement of Hedgehog signaling in specification and organization of the three tissue layers

Winata et al., 2009

ZDB-PUB-090511-18
PMID:19422819