FIGURE

Fig. 7

ID

ZDB-FIG-080715-8

Publication

Mei et al., 2008 - C1q-like inhibits p53-mediated apoptosis and controls normal hematopoiesis during zebrafish embryogenesis

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Fig. 7

The effects of C1q-like knockdown on primitive hematopoiesis. (A) Whole in situ hybridization of three hematopoietic marker genes gata-1 (a, b), scl (c, d) and hbae1 (e, f) in control MO embryos (a, c, e) and in the C1q-like morphant embryos (b, d, f) at 24 hpf. (B) Whole in situ hybridization of the hematopoietic marker gene hbae1 in control MO embryos (a, b) and in the C1q-like morphant embryos (c, d) at 48 hbf. The hybridization experiments were performed for 3 times, and at least 30 embryos were used for each experiment. The embryos are viewed at lateral views (a, c) and ventral views (b, d). The arrows indicate in situ hybridization signal. (C) Q-RT-PCR analysis of the expression changes of scl, gata-1 and hbae1 in 24 hpf and 48 hpf embryos injected with control MO or C1q-like MO. Results represent mean ± SD of three separate experiments. **p < 0.01 and ***p < 0.001.

Expression Data

Genes:	gata1a hbae1.1 tal1
Fish:	WT WT + MO1-c1ql4l
Knockdown Reagent:	MO1-c1ql4l
Anatomical Term:	intermediate cell mass of mesoderm
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-c1ql4l
Knockdown Reagent:	MO1-c1ql4l
Observed In:	primitive hemopoiesis
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 319(2), Mei, J., Zhang, Q.Y., Li, Z., Lin, S., and Gui, J.F., C1q-like inhibits p53-mediated apoptosis and controls normal hematopoiesis during zebrafish embryogenesis, 273-284, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.