FIGURE

Fig. 3

ID
ZDB-FIG-080331-13
Publication
Volkmann et al., 2008 - The zebrafish cerebellar rhombic lip is spatially patterned in producing granule cell populations of different functional compartments
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Fig. 3

gata1:GFP cells in the cerebellum are granule neurons. (A) Nucleotide and deduced amino acid sequence of isolated zebrafish gabaARα6-cDNA fragment. The characteristic subunit-specific Arginine-residue conserved throughout vertebrates is demarcated by a red box. (B) mRNA in situ hybridization to analyze gabaARα6-expression in zebrafish larvae at 7 dpf. In single optical sections recorded by confocal microscopy, 7 dpf larvae of the gata1:GFP strain display a close overlap of gabaARα6- and GFP-expression in the cerebellum [a-c, dorsolateral view to simultaneously show GFP-expressing cells in the dorsal (orange box) and the ventrolateral (blue box) cerebellum, respectively]. Magnifications of the developing dorsal cerebellum (d-f) and the ventrolateral cerebellum (g-i) demonstrate an almost complete overlap of gabaARα6- and GFP-expression. Few GFP-positive cells along the MHB (g, white arrowhead), which do not co-express gabaARα6-mRNA, may represent not yet terminally differentiated granule cells arriving after migration along the MHB. GFP-expression in zebrafish granule cells is also confined to parallel fibers (marked by white asterisk in panels b, c, e, f, h, i; see also Fig. 6), and thus, shows no co-localization with gabaARα6-mRNA expression. Abbr.: cb, cerebellum; MHB, midbrain-hindbrain boundary; rh, rhombencephalon.

Expression Data
Genes:
Fish:
Anatomical Terms:
Stage: Days 7-13

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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Reprinted from Developmental Biology, 313(1), Volkmann, K., Rieger, S., Babaryka, A., and Köster, R.W., The zebrafish cerebellar rhombic lip is spatially patterned in producing granule cell populations of different functional compartments, 167-180, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.