FIGURE

Fig. S11

ID

ZDB-FIG-080325-129

Publication

Lachnit et al., 2008 - Alterations of the cytoskeleton in all three embryonic lineages contribute to the epiboly defect of Pou5f1/Oct4 deficient MZspg zebrafish embryos

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Fig. S11

Comparison and rescue of the EVL lamellipodia phenotype. Animal pole view confocal z-projections of Alexa488-Phalloidin stained EVL cells at 90% epiboly, depicted using the depth coding method. (A) WT; (B) MZspg^hi349; (C) MZspg^m793. Extensive lamellipodia formation is observed in both alleles. (D–F) Depicts a rescue experiment showing (D) WT, (E) MZspg^hi349 non-injected, and (F) MZspg^hi349 injected with 50 pg pou5f1 mRNA. Genetically MZspg^hi349 mutant embryos are completely rescued by pou5f1 mRNA injection with respect to their EVL lamellipodia phenotype. Scale bar: 20 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pou5f3^{hi349Tg/hi349Tg} pou5f3^m793/m793 (AB)
Observed In:	EVL blastoderm cell EVL lamellipodium
Stage:	90%-epiboly

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 315(1), Lachnit, M., Kur, E., and Driever, W., Alterations of the cytoskeleton in all three embryonic lineages contribute to the epiboly defect of Pou5f1/Oct4 deficient MZspg zebrafish embryos, 1-17, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.