FIGURE

Fig. 3

ID

ZDB-FIG-071204-27

Publication

Li et al., 2007 - Redundant activities of Tfap2a and Tfap2c are required for neural crest induction and development of other non-neural ectoderm derivatives in zebrafish embryos

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Fig. 3

tfap2a/c deficient embryos display non-additive defects in pigmentation and jaw morphology. (A–D) Dorsal views of live 72 hpf embryos. (A) Melanophores are abundant on the dorsal aspect of the head in a control-MO injected wild-type embryo, slightly reduced in panel B, a control MO-injected tfap2a mutant, normal in panel C, a tfap2c MO-injected wild-type embryo, but absent from panel D, a tfap2a^-/c^MO embryo (6 of 6 tfap2a^-/c^MO embryos). (E–H) Ventral views of 4 dpf embryos processed to reveal cartilage (alcian green), and bleached to remove pigment (except panel H). (E) Cartilages derived from mandibular (1st arch, m), hyoid (2nd arch, h), and ceratobranchial (3rd–7th arches, cb) arches are seen in a control embryo. Pectoral fin (pf) is also visible. (F) A control MO-injected tfap2a homozygous mutant embryo, with normal sized mandibular, but severely reduced hyoid and ceratobranchial cartilages, as previously reported (Schilling et al., 1996). (G) A tfap2c MO-injected wild-type embryo, in which all craniofacial cartilages appear normal. (H) A tfap2a^-/c^MO embryo, in which ventral craniofacial cartilage elements and pectoral fin are absent (10 of 10 tfap2a^-/c^MO embryos). (I) Ventral view of dissected neurocranium from a control embryo. (J) Ventral view of a tfap2a^-/c^MO embryo, shown at higher magnification, only the mesoderm-derived posterior neurocranium is visible (10 of 10 tfap2a^-/c^MO embryos). e, ethmoid plate; pch, parachordal; t, trabeculae cranii (Schilling et al., 1996). Scale bars: 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tfap2a^ts213/ts213 WT + MO2-tfap2c tfap2a^ts213/ts213 + MO2-tfap2c
Knockdown Reagent:	MO2-tfap2c
Observed In:	ceratobranchial cartilage cranial cartilage melanocyte pectoral fin pharyngeal arch 2 skeleton pharyngeal arch cartilage
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 304(1), Li, W., and Cornell, R.A., Redundant activities of Tfap2a and Tfap2c are required for neural crest induction and development of other non-neural ectoderm derivatives in zebrafish embryos, 338-354, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.