FIGURE

Fig. 5

ID

ZDB-FIG-260322-28

Publication

Bakker-van Bugnum et al., 2026 - A zebrafish multimodal toolbox to study the blood-brain barrier in health and disease

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Fig. 5

Imaging-based methods to analyze brain endothelial junctions in hyperglycemic zebrafish larvae (a) Schematic figure to illustrate the orientation of the zebrafish head for images shown in (b). (b) Representative maximum intensity projection of a confocal z-stack showing the midbrain (dorsal view) in Tg(cldn5a: EGFP) zebrafish larvae at 5 dpf to visualize claudin-5 expression (green) after 48 h in 0 mM glucose control, 130 mM mannitol control or 130 mM glucose. Lower panel, higher magnification of inset (midbrain boxed in images). (c, d) High-magnification TEM images of interendothelial junctions, indicated with arrowheads. The yellow arrowhead indicates an inter-endothelial junction with increased electron-lucent space. Scale bars: 100 μm in b; scale bars: 500 nm in c, d.

Expression Data

Gene:	EGFP
Fish:	vum2Tg/vum2Tg
Condition:	chemical treatment by environment: glucose
Anatomical Term:	brain vasculature
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	vum2Tg/vum2Tg y1Tg/y1Tg
Condition:	chemical treatment by environment: glucose
Observed In:	brain vasculature midbrain tight junction
Stage:	Day 5

Phenotype Detail

Acknowledgments

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