ZFIN Image: Bakker-van Bugnum et al., 2026, Fig. 5

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Fig. 5

Imaging-based methods to analyze brain endothelial junctions in hyperglycemic zebrafish larvae (a) Schematic figure to illustrate the orientation of the zebrafish head for images shown in (b). (b) Representative maximum intensity projection of a confocal z-stack showing the midbrain (dorsal view) in Tg(cldn5a: EGFP) zebrafish larvae at 5 dpf to visualize claudin-5 expression (green) after 48 h in 0 mM glucose control, 130 mM mannitol control or 130 mM glucose. Lower panel, higher magnification of inset (midbrain boxed in images). (c, d) High-magnification TEM images of interendothelial junctions, indicated with arrowheads. The yellow arrowhead indicates an inter-endothelial junction with increased electron-lucent space. Scale bars: 100 μm in b; scale bars: 500 nm in c, d.

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Acknowledgments

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