Fig. 1

Endogenous glucose level measurements at 5 dpf in zebrafish after exogenous glucose exposure.(a) Experimental design for internal glucose measurements. Tg(fli1:EGFP) zebrafish were exposed to 60 mM or 130 mM glucose between 3 dpf and 5 dpf. 0 mM glucose and 130 mM mannitol were included as controls. Forty zebrafish from each experimental group were collected, washed and homogenized. Supernatant was collected after centrifugation and internal glucose levels were measured with a glucose meter. Images adapted from Servier Medical Art (https://smart.servier.com/), licensed under CC BY 4.0 (https://creativecommons.org/licenses/by/4.0/) and https://Biorender.com. Internal glucose levels were measured after (b) 24 h (4 dpf) or (c) 48 h (5 dpf) of exposure to 60 mM or 130 mM glucose. 0 mM glucose and 130 mM mannitol were included as controls. n = 9–16 biological replicates per group. Data are represented as mean ± SD. *P < 0.05, **P < 0.01, ****P < 0.0001 by Kruskal-Wallis statistical test.