Fig 2

Irresistible^p173 is an allele of the Cadherin-encoding gene cdh16.

(A) Conceptual translation of cdh16 and the predicted consequences of the irresistible^p173, and cdh16^co79 mutations. (B)cdh16^co79 mutants (n = 39) have decreased thresholds to low intensity acoustic stimuli as compared to their siblings (n = 33) (*p = 0.0319, ****p < 0.0001, two-way ANOVA with Šídák’s multiple comparisons test). (C)cdh16^co79 mutants (n = 39) continue responding to repeated acoustic stimuli while their siblings (n = 33) habituate. (D)cdh16^co79 mutants (n = 39) have significantly impaired habituation (****p < 0.0001, Mann–Whitney test) compared to siblings (n = 32). (E)cdh16^p173/cdh16^co79 transheterozygotes (n = 22) have increased sensitivity to acoustic stimuli when compared to cdh16^p173 heterozygotes (n = 17), cdh16^co79 heterozygotes (n = 14), and wild types (n = 16). A two-way ANOVA with Tukey’s multiple comparisons test was used to calculate the difference in SLC% between all groups. Differences between cdh16^p173/co79 versus WT (+/+) are represented with p values on the plot: ***p = 0.0005, for the difference between WT and transheterozygotes, *p < 0.03, **p = 0.0086. (F)cdh16^p173/cdh16^co79 transheterozygotes (n = 19) fail to habituate to high intensity acoustic stimuli while wild type (n = 17), cdh16^co79 heterozygotes (n = 10), and cdh16^p173 heterozygotes (n = 26) habituate normally. (G)cdh16^p173/cdh16^co79 transheterozygotes (n = 19) have significantly lower habituation percentages p < 0.0001 compared to wild types (n = 17), cdh16^co79 heterozygotes (n = 10), and cdh16^p173 heterozygotes (n = 25). Differences in habituation between groups were calculated using a two-way ANOVA with Tukey’s multiple comparisons test. Error bars in B, C, E, and F indicate SEM. Error bars in D and G indicate SD. The data underlying this figure can be found in S1 Data.