Fig 7

Putative Jun targets display varied expression patterns during larval optic nerve regeneration in control and Jun knockdown conditions.

(A–H) RT-qPCR of jun and putative Jun target genes showing average expression during regeneration in Tg(isl2b:GFP) progeny (green lines) and Tg(isl2b:GFP) X Tg(mke15Tg) DN-Jun(+), Jun knockdown, progeny (orange lines). I: Heatmap showing difference in average fold change of jun and its putative targets’ expression between Tg(isl2b:GFP) and DN-Jun(+) conditions. A–H. Average fold change of three individual replicates were plotted for each condition and timepoint. Each timepoint required n =  90 retinas (30 retinas per replicate for 3 replicates) for both Tg(isl2b:GFP) and DN-Jun(+) experiments. Student’s T-Test was used to determine statistical significance between control and knockdown conditions for each timepoint. *  =  p < 0.05, ** =  p < 0.01, *** =  p < 0.001, **** =  p < 0.0001. All transcripts and primers for these data are shown in Table 1 and transcript ID can be found in S2 Table.