Quantification of COS length and number of phagosomes per COS in abca4 mutant fish aged 2 month or 1 year. (A,B) Lengths measurements of individual cone outer segment (COS) subtypes in retinas obtained from 2-month-old (A) and 1-year-old (B) wild-type (wt) or abca4aca30/ca30 (abca4a−/−), abca4bca33/ca33 (abca4b−/−), abca4aca30/ca30;abca4bca33/ca33 (abca4aca30/ca30;abca4a−/−) and abca4aca31/ca31;abca4bca33/ca33 (abca4aca31/ca31;abca4b−/−) mutant zebrafish. Data for each genotype are combined measurements from at least three eyes obtained from individual fish (individual measurements are provided in Fig. S2). Solid horizontal lines represent the mean; dashed horizontal lines indicate quartiles. (C,D) Numbers of shed COS phagosomes were normalized to the number of COS for each cone type in 2-month-old (C) or 1-year-old (D) wild-type (wt) or abca4aca30/ca30, abca4b−/−, abca4aca30/ca30;abca4bca33/ca33 and abca4aca31/ca31;abca4bca33/ca33 mutant fish. Each symbol represents data from an image from an eye from an individual fish; horizontal line represents the mean and vertical error bars indicate the s.e.m. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 (one-way ANOVA, significance by Tukey's multiple comparisons test).
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