Comparison of raraa and rarab gene expression in sox17:eGFP transgenic embryos. HCR shows the expression of raraa (A, A′, B, B′, C, C′, D, D′, E, E′) and rarab (F, F′, G, G′, H, H′, I, I′, J, J′) genes from 12 hpf to 32 hpf in relation to sox17:eGFP expression. Genes raraa and rarab are co-expressed with sox17 at all time points examined. However, raraa is only co-expressed with sox17 at the tail of the embryos at time points 18 hpf and 24 hpf. A, A′, F, F’: Lateral views at 12 hpf. B, B′, G, G’: Lateral views at 16 hpf. C, C′, H, H’: Lateral views at 18 hpf. D, D′, I, I’: Lateral views of 24 hpf embryo head. E, E′,J, J’: Lateral views of 32 hpf embryo head. For all panels, green labels sox17:eGFP. For panels A–E′, magenta and grayscale images label raraa expression. For panels F–J′ magenta and grayscale images label rarab expression. Arrows indicate co-expression between sox17:eGFP and rara genes. The asterisks label the position of the otic vesicle. represent Scale Bar represents: 100um for images A-C′, F-I′ and 100um for images D-E′, I-J’. The inserts magnify the co-expression between the pharyngeal endoderm and the genes shown.
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