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Comparison of rxrba and rxrbb gene expression in and sox10:eGFP transgenic embryos. HCR shows the expression of rxraa (A, A′, B, B′, C, C′, D, D′) and rxrab (E, E′ F, F′, G, G′, H, H′) genes from 16 hpf to 32 hpf within sox10:GFP transgenic embryos. Both rxrba and rxrbb are highly expressed throughout the whole embryo. A, A′, E, E’: Lateral views at 16 hpf. B, B′, F, F’: Lateral views at 18 hpf. C, C′, G, G’: Lateral views of 24 hpf embryo head. D, D′, H, H’: Lateral views of 32 hpf embryo head. For all panels, green labels sox10:eGFP. For panels A–D′, magenta and grayscale images label rxrba expression. For panels E–H′ magenta and grayscale images label rxrbb expression Arrows indicate co-expression between sox10:eGFP and rxrb genes. The asterisks label the position of the otic vesicle. Scale Bar represents: 100um for images A-H’. The inserts magnify the co-expression between the neural crest and the genes shown.
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