Figure 5

Pharmacological inhibition of ATM activation mitigates the enhanced UV radiation-induced effects after Brcc3 depletion. (A) Model for ATM or ATR in Response to DNA Damage. (B) Schematic diagram of qRT-PCR or phenotypic analysis of embryos after UV radiation with/without ATM inhibitors. WT and brcc3 mutant embryos at 22 hpf with/without ATM inhibitors treated were raised to 24 hpf, treated with UV radiation for 30 s, and then subjected for qRT-PCR or phenotypic analysis at indicated timepoint. (C) Representative images of UV radiation-treated WT and brcc3 mutant embryos at 48 hpf in addition of different doses of ATM/ATR inhibitors. Scale bar = 1 mm. (D) The viability of WT and brcc3 mutant embryos in (B). Values are represented as means ± s.d. ns, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001. (one-way ANOVA followed by Tukey’s post hoc test). (E) The relative mRNA levels of p53 and its target genes in UV radiation-treated WT and brcc3 mutant embryos at 28 hpf with/without KU60019 inhibitor. Results are from three independent replicates. Values are represented as means ± s.d. ns, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001. (one-way ANOVA followed by Tukey’s post hoc test).