FIGURE

Fig. 6.

ID

ZDB-FIG-240304-24

Publication

Hewitt et al., 2024 - Spherical harmonics analysis reveals cell shape-fate relationships in zebrafish lateral line neuromasts

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Fig. 6.

Location and shape characteristics of cells expressing sost:NLS-Eos and sfrp1a:NLS-Eos. (A) Single confocal slice of a neuromast from sost:NLS-Eos; Tg(-8.0cldnb:LY-EGFP) fish stained with DRAQ5. Image contrast was adjusted for visibility. (B) The same slice as in A, with the sost:NLS-Eos channel (gray) overlaid with a mask indicating cells classified as Eos⁺ (cyan). (C) Polar plot showing relative cell locations of locations of Eos⁺ (cyan) and Eos⁻ (gray) cells. (D) UMAP of Eos⁺ (cyan) and Eos⁻ (gray) cells from sost:NLS-Eos. (E) sost:NLS-Eos⁺ cells plotted as raw counts within each cell shape cluster. (F) sost:NLS-Eos⁺ cells plotted as the percentage of each cluster. (G) Single confocal slice of a neuromast from sfrp1a:NLS-Eos; Tg(-8.0cldnb:LY-EGFP) fish stained with DRAQ5. Image contrast was adjusted for visibility. (H) The same slice as in A, with the sfrp1a:NLS-Eos channel (gray) overlaid with a mask indicating cells classified as Eos⁺ (cyan). (I) Polar plot showing relative cell locations of locations of Eos⁺ (cyan) and Eos⁻ (gray) cells. (J) UMAP of Eos⁺ (cyan) and Eos⁻ (gray) cells from sfrp1a:NLS-Eos. (K) sfrp1a:NLS-Eos⁺ cells plotted as raw counts within each cell shape cluster. (L) sfrp1a:NLS-Eos⁺ cells plotted as the percentage of each cluster.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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