PUBLICATION
Spherical harmonics analysis reveals cell shape-fate relationships in zebrafish lateral line neuromasts
- Authors
- Hewitt, M.N., Cruz, I.A., Raible, D.W.
- ID
- ZDB-PUB-240127-10
- Date
- 2024
- Source
- Development (Cambridge, England) 151(2): (Journal)
- Registered Authors
- Raible, David
- Keywords
- Hair cell, Lateral line, Zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cell Shape
- Hair Cells, Auditory/physiology
- Lateral Line System*
- Zebrafish*/genetics
- PubMed
- 38276966 Full text @ Development
Citation
Hewitt, M.N., Cruz, I.A., Raible, D.W. (2024) Spherical harmonics analysis reveals cell shape-fate relationships in zebrafish lateral line neuromasts. Development (Cambridge, England). 151(2):.
Abstract
Cell shape is a powerful readout of cell state, fate and function. We describe a custom workflow to perform semi-automated, 3D cell and nucleus segmentation, and spherical harmonics and principal components analysis to distill cell and nuclear shape variation into discrete biologically meaningful parameters. We apply these methods to analyze shape in the neuromast cells of the zebrafish lateral line system, finding that shapes vary with cell location and identity. The distinction between hair cells and support cells accounted for much of the variation, which allowed us to train classifiers to predict cell identity from shape features. Using transgenic markers for support cell subpopulations, we found that subtypes had different shapes from each other. To investigate how loss of a neuromast cell type altered cell shape distributions, we examined atoh1a mutants that lack hair cells. We found that mutant neuromasts lacked the cell shape phenotype associated with hair cells, but did not exhibit a mutant-specific cell shape. Our results demonstrate the utility of using 3D cell shape features to characterize, compare and classify cells in a living developing organism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping