Fig. 8

otpa and sim1a expression is reduced in the H/PT region upon loss of either Neurog1 or Olig2. (A–D) otpa expression in (B,D) neurog1hi1059/hi1059 mutants compared to (A,C) wildtype siblings at (A,B) 30 hpf and (C,D) 48 hpf. In neurog1hi1059/hi1059 mutants otpa expression in the H/PT is reduced if compared to wildtype siblings (black arrowheads). (E–H) otpa expression in (F,H) olig2m1317/m1317 mutants compared to (E,G) wildtype siblings at (E,F) 30 hpf and (G,H) 48 hpf. At 30 hpf in (F) olig2m1317/m1317 mutants, the otpa expression in the H/PT is reduced compared to (E) wildtype (black arrowhead). At 48 hpf in (H) olig2m1317/m1317 mutants otpa expression in the caudal H/PT is reduced (black arrowheads) and unchanged in the rostral H/PT (non-filled arrowheads). (I–L) sim1a expression in (J,L) neurog1hi1059/hi1059 mutants compared to (I,K) wildtype siblings at (I,J) 30 hpf and (K,L) 48 hpf. In neurog1hi1059/hi1059 mutants sim1a expression in the H/PT is reduced if compared to wildtype siblings at 30 hpf but not at 48 hpf (black arrowheads). (M–P) sim1a expression in (N,P) olig2m1317/m1317 mutants compared to (M,O) wildtype siblings at (M,N) 30 hpf and (O,P) 48 hpf. In olig2m1317/m1317 mutants, the sim1a expression in the H/PT is reduced compared to wildtype at 30 hpf as well as 48 hpf (black arrowheads). The phenotypes were observed in N/N (number of embryos with phenotype shown in figure panel/total number of embryos analyzed): (A) 10/10; (B) 15/15; (C) 9/9; (D) 12/12; (E) 18/18: (F) 12/12; (G) 17/17; (H) 6/6; (I) 6/6; (J) 7/7; (K) 5/5; (L) 10/10; (M) 4/4; (N) 11/11; (O) 3/3; (P) 6/6. Abbreviations: H, hypothalamus; PO, preoptic area; PT, posterior tuberculum. All images show lateral views (48 hpf: enucleated embryos) and are single planes. Anterior is to the left. Scale bar: 100 μm.