PUBLICATION
Neurog1 and Olig2 integrate patterning and neurogenesis signals in development of zebrafish dopaminergic and glutamatergic dual transmitter neurons
- Authors
- Altbürger, C., Rath, M., Armbruster, D., Driever, W.
- ID
- ZDB-PUB-231110-2
- Date
- 2023
- Source
- Developmental Biology 505: 859885-98 (Journal)
- Registered Authors
- Driever, Wolfgang
- Keywords
- Dopaminergic neuron, Neurog1, Neurogenesis, Notch signaling, Olig2, bHLH factor
- MeSH Terms
-
- Gene Expression Regulation, Developmental
- Animals
- Basic Helix-Loop-Helix Transcription Factors/genetics
- Basic Helix-Loop-Helix Transcription Factors/metabolism
- Mammals/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Transcription Factors/metabolism
- Zebrafish*/genetics
- Cell Differentiation
- Neurogenesis/physiology
- Neurons*/metabolism
- PubMed
- 37944224 Full text @ Dev. Biol.
Citation
Altbürger, C., Rath, M., Armbruster, D., Driever, W. (2023) Neurog1 and Olig2 integrate patterning and neurogenesis signals in development of zebrafish dopaminergic and glutamatergic dual transmitter neurons. Developmental Biology. 505:859885-98.
Abstract
Dopaminergic neurons develop in distinct neural domains by integrating local patterning and neurogenesis signals. While the proneural proteins Neurog1 and Olig2 have been previously linked to development of dopaminergic neurons, their dependence on local prepatterning and specific contributions to dopaminergic neurogenesis are not well understood. Here, we show that both transcription factors are differentially required for the development of defined dopaminergic glutamatergic subpopulations in the zebrafish posterior tuberculum, which are homologous to A11 dopaminergic neurons in mammals. Both Olig2 and Neurog1 are expressed in otpa expressing progenitor cells and appear to act upstream of Otpa during dopaminergic neurogenesis. Our epistasis analysis confirmed that Neurog1 acts downstream of Notch signaling, while Olig2 acts downstream of Shh, but upstream and/or in parallel to Notch signaling during neurogenesis of A11-type dopaminergic clusters. Furthermore, we identified Olig2 to be an upstream regulator of neurog1 in dopaminergic neurogenesis. This regulation occurs through Olig2-dependent repression of the proneural repressor and Notch target gene her2. Our study reveals how Neurog1 and Olig2 integrate local patterning signals, including Shh, with Notch neurogenic selection signaling, to specify the progenitor population and initiate neurogenesis and differentiation of A11-type dopaminergic neurons.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping