Fig. 5
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- ZDB-FIG-240229-27
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- Altbürger et al., 2023 - Neurog1 and Olig2 integrate patterning and neurogenesis signals in development of zebrafish dopaminergic and glutamatergic dual transmitter neurons
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Analysis of potential cross-regulation of neurog1 and olig2 expression. (A–D) olig2 expression in (B,D) neurog1hi1059/hi1059 mutants compared to (A,C) wildtype siblings at (A,B) 30 hpf and (C,D) 48 hpf. At 48 hpf, olig2 expression in the hindbrain is reduced in (D) neurog1hi1059/hi1059 mutants compared to (C) wildtype siblings (transparent arrowheads). (E–H) neurog1 expression in (F,H) olig2m1317/m1317 mutants compared to (E,G) wildtype siblings at (E,F) 30 hpf and (G,H) 48 hpf. At 30 and 48 hpf, neurog1 expression in the H/PT is reduced in (F,H) olig2m1317/m1317 mutants compared to (E,G) wildtype siblings (black arrowheads). (I–L) neurog1 expression upon heat-shock in (J,L) Tg(hsp70l:olig2)m1306 transgenic fish compared to (I,K) wildtype siblings. (K,L) are magnifications of (I,J) respectively. (J,L) Tg(hsp70l:olig2)m1306 transgenic fish show increased neurog1 expression in the H/PT when compared to (I,J) wildtype siblings (arrowheads). All olig2 overexpressing embryos of each genotype showed increased neurog1 expression in hypothalamus and posterior tuberculum. The phenotypes were observed in N/N (number of embryos with phenotype shown in figure panel/total number of embryos analyzed): (A) 8/8; (B) 6/6; (C) 10/10; (D) 7/7; (E) 7/7: (F) 7/7; (G) 10/10; (H) 8/8; (I,K) 8/8; (J,L) 8/8. Abbreviations: H, hypothalamus; HB, hindbrain; PT, posterior tuberculum; Tel, telencephalon. All images show lateral views (C,D and G,H show enucleated embryos) and single optical planes. Anterior is to the left. Scale bars: 100 μm. |
Reprinted from Developmental Biology, 505, Altbürger, C., Rath, M., Armbruster, D., Driever, W., Neurog1 and Olig2 integrate patterning and neurogenesis signals in development of zebrafish dopaminergic and glutamatergic dual transmitter neurons, 859885-98, Copyright (2023) with permission from Elsevier. Full text @ Dev. Biol.