Fig. 3

Heat-shock driven overexpression of neurog1 or olig2 induces supernumerary and ectopic DA neurons. th expression was analyzed following heat-shock in (B,D,F) Tg(hsp70:neurog1)ups1 compared to (A,C,E) wildtype siblings at (A,B) 30 hpf and (C–F) 72 hpf. (G–L) th expression in heat shocked (H,J,L) Tg(hsp70l:olig2)m1306 compared to (G,I,K) wildtype siblings at (G,H) 30 hpf and (I–L) 72 hpf. The numbers 1 through 6 mark the dopaminergic cell clusters DAC1-DAC6 (E). Overexpression of neurog1 induces supernumerary and ectopic th+ cells at 30 hpf and 72 hpf (B,D,F). Overexpression of olig2 results in supernumerary th+ cells at 30 hpf and 72 hpf, probably belonging to the DAC2 group (H,J,L). (M) Overview of effects of neurog1 and olig2 mutant and overexpression, as well as of Notch inhibition on the development of catecholaminergic neurons in the specific anatomical clusters. The specific experiments are shown in Fig. 1, Fig. 2 and this Figure. CC indicates that cells of the indicated CA groups were counted. EN indicates enhanced stain intensity or ectopic catecholaminergic neurons, which were not counted. Red colored boxes indicate significantly decreased numbers of CA neurons whereas green colored boxes indicate significantly increased cell numbers. Grey colored boxes indicate no change in cell numbers between the given genotypes and treatment conditions. For numbers see Supplemental Table 3. Abbreviations: numbers 1 through 6 for DAC1-6, dopaminergic cell cluster 1–6; H, hypothalamus; LC, locus coeruleus; PO, preoptic area; Pr, pretectum; PT, posterior tuberculum; TC, telencephalic cluster. (A-D, G-J) show lateral views (I,J show enucleated embryos) and (E,F,K,L) dorsal views. All images show single optical planes. Anterior is to the left. Scale bars: 100 μm.