FIGURE

Figure 5

ID

ZDB-FIG-240229-194

Publication

Zhang et al., 2024 - Nomo1 deficiency causes autism-like behavior in zebrafish

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Figure 5

Loss-of-function of nomo1 affected neurodevelopment in larval zebrafish.

(A, B) EGFP labeled GABAergic inhibitory neurons and dsRed labeled glutamatergic excitatory neurons at 48 hpf and 3 dpf of WT and nomo1^−/−. Scale bar=1 mm. (C, D) RFP labeled neuro progenitors at 3 dpf and 6 dpf of WT and nomo1^−/−. Scale bar=1 mm. (E, F) Quantitative analysis of fluorescent signal (biological replicates, n = 4). (G) The relative expression level of neurological genes in nomo1^−/− brain at 3 mpf (N = 4 × 5 animals per group). (H) The relative expression level of apoptosis genes in nomo1^−/− brain at 3 mpf (N = 4 × 5 animals per group). Data information: Data are analyzed using unpaired t test and presented as the means ± SEM. ^*P < 0.05 and ^**P < 0.01, ^****P < 0.0001. Source data are available online for this figure.

Expression Data

Genes:	bbc3 bcl2a bcl2l1 dlx2a DsRed EGFP egr2b elavl3 fezf2 foxb1a gad1b isl1a neurog1 pidd1 RFP sim1a (all 16)
Fish:	nomo^{zf3952/zf3952} nomo^{zf3952/zf3952}; fdu101Tg/fdu101Tg nomo^{zf3952/zf3952}; ion84Tg/ion84Tg nomo^{zf3952/zf3952}; nns14Tg/nns14Tg
Anatomical Terms:	brain forebrain GABAergic neuron forebrain glutamatergic neuron hindbrain GABAergic neuron hindbrain glutamatergic neuron midbrain GABAergic neuron midbrain glutamatergic neuron neuronal stem cell spinal cord GABAergic neuron spinal cord glutamatergic neuron (all 10)
Stage Range:	Long-pec to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nomo^{zf3952/zf3952} nomo^{zf3952/zf3952}; fdu101Tg/fdu101Tg nomo^{zf3952/zf3952}; ion84Tg/ion84Tg nomo^{zf3952/zf3952}; nns14Tg/nns14Tg
Observed In:	brain forebrain GABAergic neuron forebrain glutamatergic neuron hindbrain GABAergic neuron hindbrain glutamatergic neuron midbrain GABAergic neuron midbrain glutamatergic neuron neuronal stem cell spinal cord GABAergic neuron spinal cord glutamatergic neuron (all 10)
Stage Range:	Long-pec to Adult

Phenotype Detail

Acknowledgments

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