FIGURE

Fig. 3

ID

ZDB-FIG-240206-3

Publication

Zhao et al., 2023 - Glycosylated queuosines in tRNAs optimize translational rate and post-embryonic growth

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

In vitro reconstitution and kinetic analysis of Q-glycosylation (A) In vitro reconstitution of galQ34 in human tRNATyr with recombinant QTGAL in the presence (+) or absence (−) of UDP-gal. (B) In vitro reconstitution of manQ34 in E. coli tRNAAsp with recombinant QTMAN in the presence (+) or absence (−) of GDP-man. (C and D) Kinetic analyses of galQ34 (C) or manQ34 (D) formation by recombinant QTGAL or QTMAN with different concentrations of human tRNATyr or E. coli tRNAAsp (upper) and UDP-gal or GDP-man (lower), respectively. The initial velocity of galQ34 or manQ34 formation (nM/s) is plotted as the mean ± standard deviation (SD; n = 3). Data are fitted to the Michaelis-Menten equation to yield Km and Vmax values (inset). See also Figures S1, S10, and S11.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Cell, 186(25), Zhao, X., Ma, D., Ishiguro, K., Saito, H., Akichika, S., Matsuzawa, I., Mito, M., Irie, T., Ishibashi, K., Wakabayashi, K., Sakaguchi, Y., Yokoyama, T., Mishima, Y., Shirouzu, M., Iwasaki, S., Suzuki, T., Suzuki, T., Glycosylated queuosines in tRNAs optimize translational rate and post-embryonic growth, 5517-5535.e24, Copyright (2023) with permission from Elsevier. Full text @ Cell