Figure 4

Canthin-6-one inhibits tumour-associated angiogenesis in zebrafish. (A–C) Lateral light-sheet microscope images of 2 dpt (4 dpf) Tg(fli1a:EGFP) larvae injected with B16F10 melanoma cells and treated with either 0.2% DMSO (A), 20 µM sunitinib malate (SM, B), or 20 µM canthin-6-one (C) from 2 dpf. White arrow heads show tumour-associated blood vessels and asterisks indicate the absence of tumour-associated angiogenesis. (D,E) Quantification of blood vessels within the B16F10 tumour mass relative to tumour mass volume (D) or tumour mass volume (E) in 2 dpt (4 dpf) Tg(fli1a:EGFP) larvae injected with B16F10 melanoma cells and treated with either 0.2% DMSO (n = 38 larvae), 20 µM SM (n = 23 larvae), or 20 µM canthin-6-one (n = 30 larvae). Statistical test: Ordinary one-way ANOVA test was conducted for graphs (D,E). p ≤ 0.0001 (****), p ≤ 0.05 (*), p > 0.05 (not significant, n.s.). Scale bar: 100 µm (A).