FIGURE

Figure 2.

ID

ZDB-FIG-231002-64

Publication

Gao et al., 2023 - Sensory deficit screen identifies nsf mutation that differentially affects SNARE recycling and quality control

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Figure 2.

The I209N mutation in Nsfa selectively affects hearing and vestibular function without causing paralysis

(A) Still frames from high-speed videos of larvae. nsfa^st53 homozygotes were paralyzed and unresponsive to touch, whereas nsfa^I209N homozygotic and nsfa^I209N/st53 larvae displayed robust startle reflexes.

(B) (B and C) Acoustic evoked behavioral responses of larvae exposed to a 600-Hz stimulus at the intensities indicated normalized to homozygous WT siblings. n indicates number of fish tested.

(D–K) Vestibulospinal reflexes are reduced in nsfa^I209N homozygotic and nsfa^I209N/st53 larvae. (E–G) Raw traces (blue) of the tail movements of representative WT and mutant larvae. The rolling median (orange trace) is a movement artifact. (H–K) The maximum tail angle (H and I) and the normalized integral (J and K) of sibling cohorts were quantified using ZebraZoom. n indicates number of fish tested.

Mean ± SEM and two-way ANOVA with Benjamini-Hochberg correction for each dataset were performed. *p < 0.05, **p < 0.01, ***p < 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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