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Figure 2.

ID
ZDB-IMAGE-231002-62
Source
Figures for Gao et al., 2023
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Figure Caption

Figure 2. The I209N mutation in Nsfa selectively affects hearing and vestibular function without causing paralysis

(A) Still frames from high-speed videos of larvae. nsfast53 homozygotes were paralyzed and unresponsive to touch, whereas nsfaI209N homozygotic and nsfaI209N/st53 larvae displayed robust startle reflexes.

(B) (B and C) Acoustic evoked behavioral responses of larvae exposed to a 600-Hz stimulus at the intensities indicated normalized to homozygous WT siblings. n indicates number of fish tested.

(D–K) Vestibulospinal reflexes are reduced in nsfaI209N homozygotic and nsfaI209N/st53 larvae. (E–G) Raw traces (blue) of the tail movements of representative WT and mutant larvae. The rolling median (orange trace) is a movement artifact. (H–K) The maximum tail angle (H and I) and the normalized integral (J and K) of sibling cohorts were quantified using ZebraZoom. n indicates number of fish tested.

Mean ± SEM and two-way ANOVA with Benjamini-Hochberg correction for each dataset were performed. *p < 0.05, **p < 0.01, ***p < 0.001.

Acknowledgments
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