Figure 7—figure supplement 2—source data 1.

Optogenetic control of the heart by <italic toggle='yes'>b</italic>PAC or <italic toggle='yes'>Oa</italic>PAC.

(A) Expression of bPAC-MT or OaPAC-Flag in cardiomyocytes. 4-dpf larvae expressing bPAC-MT or OaPAC-Flag were fixed and stained with anti-Myc or Flag (green), and anti-DsRed (mCherry, magenta) antibodies. (B, C) Heartbeats (HBs) monitored by luminosity (AU), changes (B), and relative HB frequency (C) of bPAC-expressing larvae. The heart area of Tg larvae expressing bPAC was irradiated with light (0.5 mW/mm2) of 470 nm for 5 s at the indicated periods. Similar results were obtained from four Tg larvae. A typical example from one larva is shown in (B), and average HB frequency of the first or second trial showing a typical pattern for the four larvae is shown in (C). The larvae showed induced bradycardia in the third through sixth trials. Scale bar = 100 μm in (A).

Data for <xref rid='fig7s2' ref-type='fig'>Figure 7—figure supplement 2</xref>, optogenetic control of the heart by <italic toggle='yes'>b</italic>PAC.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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