PUBLICATION
Optogenetic manipulation of neuronal and cardiomyocyte functions in zebrafish using microbial rhodopsins and adenylyl cyclases
- Authors
- Hagio, H., Koyama, W., Hosaka, S., Song, A.D., Narantsatsral, J., Matsuda, K., Shimizu, T., Hososhima, S., Tsunoda, S.P., Kandori, H., Hibi, M.
- ID
- ZDB-PUB-230818-45
- Date
- 2023
- Source
- eLIFE 12: (Journal)
- Registered Authors
- Hibi, Masahiko, Shimizu, Takashi
- Keywords
- adenylyl cyclase, cardiac contraction, cell biology, guanylyl cyclase, locomotion, neuroscience, optogenetics, rhodopsin, zebrafish
- MeSH Terms
-
- Adenylyl Cyclases/genetics
- Animals
- Heart Arrest*
- Myocytes, Cardiac*
- Neurons
- Optogenetics
- Rhodopsins, Microbial
- Zebrafish
- PubMed
- 37589546 Full text @ Elife
Citation
Hagio, H., Koyama, W., Hosaka, S., Song, A.D., Narantsatsral, J., Matsuda, K., Shimizu, T., Hososhima, S., Tsunoda, S.P., Kandori, H., Hibi, M. (2023) Optogenetic manipulation of neuronal and cardiomyocyte functions in zebrafish using microbial rhodopsins and adenylyl cyclases. eLIFE. 12:.
Abstract
Even though microbial photosensitive proteins have been used for optogenetics, their use should be optimized to precisely control cell and tissue functions in vivo. We exploited GtCCR4 and KnChR, cation channelrhodopsins from algae, BeGC1, a guanylyl cyclase rhodopsin from a fungus, and photoactivated adenylyl cyclases (PACs) from cyanobacteria (OaPAC) or bacteria (bPAC), to control cell functions in zebrafish. Optical activation of GtCCR4 and KnChR in the hindbrain reticulospinal V2a neurons, which are involved in locomotion, induced swimming behavior at relatively short latencies, whereas activation of BeGC1 or PACs achieved it at long latencies. Activation of GtCCR4 and KnChR in cardiomyocytes induced cardiac arrest, whereas activation of bPAC gradually induced bradycardia. KnChR activation led to an increase in intracellular Ca2+ in the heart, suggesting that depolarization caused cardiac arrest. These data suggest that these optogenetic tools can be used to reveal the function and regulation of zebrafish neurons and cardiomyocytes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping