Figure 6

Schematic overview of LC3-associations with Mm-containing vesicles. Zebrafish in vivo imaging of Mm infection using the TFI protocol allowed us to distinguish between different LC3-vesicle membrane associations upon Mm infection. Macrophages are recruited to the site of infection at <30 mpi, and initial phagocytosis is observed at 30 mpi (Figure 1). At 60 mpi, LC3-vesicle membrane associations are visible in the proximity of bacterial clusters and colocalizing with them (Figure 4). The scheme illustrates the typically observed shapes: LC3-positive compact vesicle (Figure 2), LC3 puncta (Figure 4), LC3-positive spacious vesicle (Figure 5), and LC3-positive compound vesicles (Figure 3). Around 200 mpi, cells carrying bacteria inside LC3-positive vesicles were seen migrating away from the site of infection towards the caudal vein area, possibly disseminating the infection. Such motility was not observed for cells containing large LC3-negative bacterial clusters. Figure created with BioRender.