Figure 5

Early dissemination of Mm infection. Transgenic (CMV:GFP-LC3) zebrafish larvae, labeling LC3 autophagy-related protein (green), were infected with 300 CFU of Mm-mCherry (pseudo color magenta) following the TFI protocol. Maximum projection stills are shown of the entire time-lapse field of view of the injected area, with the ROI enlarged in the insets (Supplementary Video S7). At 75 mpi, the first LC3-positive Mm-containing vesicles are seen (inset, arrowhead). ROI 2 follows a big phagocytosed bacteria cluster displaced surrounding the core infection site while fragmented into smaller clusters. At 170 mpi, clear LC3-positive spacious vesicles engulf some previously fragmented clusters (inset, arrowhead). Area 2 follows a different LC3-positive Mm cluster. At 215 mpi, the phagocytic cell moves towards the proximity of the caudal vein area at the bottom of the field of view. Scale bar: 10 μm.