FIGURE

Figure 2

ID

ZDB-FIG-230526-63

Publication

Zeng et al., 2023 - Loss of threonyl-tRNA synthetase-like protein Tarsl2 has little impact on protein synthesis but affects mouse development

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Figure 2

Tars1 is indispensable in vivo.A, statistical analysis showing the genetic features of the Tars1-KO mice (n = 34). B, body features of the 10-week-old Tars1^+/+ and Tars1^+/− mice. The scale bar represents 1 cm. C, statistical analysis showing the weights of the 16-week-old Tars1^+/+ (n = 7) and Tars1^+/− (n = 6) male mice. D, statistical analysis showing the weights of the 16-week-old Tars1^+/+ (n = 5) and Tars1^+/− (n = 4) female mice. E, protein abundance of the 8-week-old Tars1 and Tarsl2 in Tars1^+/+ and Tars1^+/− mouse muscle as detected by Western blot. Gapdh was used as the loading control. F, levels of tRNA^Ala(AGC), tRNA^Thr(AGU), and tRNA^Thr(CGU) in muscle tissues of the 8-week-old Tars1^+/+ and Tars1^+/− mice. 5S RNA was as the loading control. G, aminoacylation levels of tRNA^Thr(AGU) and tRNA^Thr(CGU) in muscle tissues of the 8-week-old Tars1^+/+ and Tars1^+/− mice. ∗, a nonspecific band. De: tRNA deaminoacylation control. Error bars in (C–G) represent the mean ± SD with the p values indicated (two-tailed Student’s t test).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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