FIGURE

Figure 2

ID

ZDB-FIG-221214-278

Publication

Zhang et al., 2022 - Requirement of Zebrafish Adcy3a and Adcy5 in Melanosome Dispersion and Melanocyte Stripe Formation

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Figure 2

adcy3a^-/^- and adcy5^-/^- double mutants show high melanosome aggregation. (A–H) adcy3a^-/^-;adcy3b^-/^- (B) and adcy6a^-/^-;adcy6b^-/^- (C) double mutants as well as adcy6a-MO-injected adcy3a^-/^- mutants (D) display normal distribution of melanosomes as that of WT embryos (A) under dark conditions at 4dpf. adcy6a-MO injected adcy5^-/^- mutants (F) exhibit comparable pigmentation as adcy5^-/^- (E). adcy3a^-/^-;adcy5^-/^- double mutants (G) develop a higher degree of aggregation of melanosomes than adcy5^-/^-, while co-injection of adcy3a and adcy5 mRNA can significantly rescue the defects in melanosome aggregation in adcy3a^-/^-;adcy5^-/^- double mutants (H). Scale bar = 100 μm in (A–H). (I) Pigmentation coverage shows no difference among wild-type embryos, adcy3a^-/^-;adcy3b^-/^-, adcy6a^-/^-;adcy6b^-/^-, and adcy3a^-/^- mutants injected with adcy6a-MO, as well as between adcy5^-/^- and adcy5^-/^- mutants injected with adcy6a-MO. adcy3a^-/^-;adcy5^-/^- double mutants have significantly reduced pigmentation areas compared to adcy5^-/^-, which can be rescued by co-injection of adcy3a and adcy5 mRNA (Student’s t-test, *** p < 0.001, **** p < 0.0001, n.s., not significant, p > 0.05). (J) Statistical analyses show that the number of melanocytes in adcy5^-/^- single mutants, and adcy3a^-/^-;adcy3b^-/^-, adcy6a^-/^-;adcy6b^-/^- and adcy3a^-/^-;adcy5^-/^- double mutants is comparable with that of WT embryos (Student’s t-test, n.s., not significant, p > 0.05). (K–R) WISH analyses showed the expression levels of sox10, mitfa, tyr, and dct are normal in adcy5^-/-;adcy3a^-/^- compared to WT embryos.

Expression Data

Genes:	dct mitfa sox10 tyr
Fish:	WT adcy3a^{zf3713/zf3713}; adcy5^{zf3715/zf3715}
Anatomical Terms:	melanoblast melanocyte
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	adcy5^{zf3715/zf3715} adcy3a^{zf3713/zf3713} + MO2-adcy6a adcy5^{zf3715/zf3715} + MO2-adcy6a adcy3a^{zf3713/zf3713}; adcy3b^{zf3718/zf3718} adcy3a^{zf3713/zf3713}; adcy5^{zf3715/zf3715} adcy6a^{zf3717/zf3717}; adcy6b^{zf3719/zf3719} (all 6)
Condition:	constant dark
Knockdown Reagent:	MO2-adcy6a
Observed In:	integument integument melanocyte integument melanosome integument melanosome localization
Stage:	Day 4

Phenotype Detail

Acknowledgments

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