Fig. 6

Figure 6. Local V1 synapses onto CoPr neurons and normalized connectivity map

(A) Schematic of the experimental design showing intracellular recording from CoPr neurons (blue) paired with optical stimulation of V1 neurons (black) along the rostro-caudal axis.

(B and C) Representative traces of IPSCs (B) and the total charge transfer (C) recorded in CoPr neurons with optical stimulation of V1 neurons along different segments in the rostro-caudal axis. Colored traces in (B) indicate mean. Red asterisks mark segments that were significantly different from noise (p < 0.05).

(D and E) Comparison of the percent of squares in the optical stimuli grid that evoked IPSCs (D) and the conductance of IPSCs (E) in CoPr neurons. n = 5–7 neurons for each point.

(F) Summary of V1 connectivity to different post-synaptic targets. Top: schematic of the inferred connectivity of V1 neurons (black) to different targets locally and distally is shown. Yellow dots symbolize inhibitory synapses. Bottom: heatmap shows normalized charge transfer for the different post-synaptic targets along the rostro-caudal axis. The charge transfer per segment for each recorded neuronal target was normalized to its measured intrinsic neuronal conductance (inverse of R_in). Median values of normalized charge transfer for each target cell population are plotted. Values for segment 4 and segment 6 were interpolated as averages of the two neighboring segments. The resulting values are plotted on the same color scale for all target populations.

See also Figure S6.