FIGURE

Fig. 9

ID

ZDB-FIG-220912-100

Publication

Atienzar-Aroca et al., 2022 - Transgenic Overexpression of Myocilin Leads to Variable Ocular Anterior Segment and Retinal Alterations Associated with Extracellular Matrix Abnormalities in Adult Zebrafish

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Fig. 9

Figure 9. Increased number of retinal ganglion cells in the retinal mass present in the most severe phenotype of old (two-year-old) myocilin transgenic zebrafish. Overview and detailed images of (A,B) wild-type zebrafish eye and (C–F) myocilin transgenic zebrafish eye. Images in (B,D,F) are details indicated by white boxes in (A,C,E), respectively. (A,B) Double immunostaining for calretinin (CR), a calcium-binding protein, and the transcription factor Brn3a was used for the identification of ganglion cells (arrowheads). Optic nerve fibers were immuno-positive for calretinin. TO-PRO-3-iodide was used for nuclei staining. (C–F) Asterisks: axon bundles. Scale bars in (A,C,E): 200 µm; scale bars in (B,D,F): 20 µm. ONL: outer nuclear layer; OPL: outer plexiform layer; INL: inner nuclear layer; IPL: inner plexiform layer; GCL: ganglion cell layer; NFL: nerve fiber layer; Tg/+: transgenic; +/+: wild type.

Expression Data

Gene:	pou4f1
Antibodies:	Ab2-pou4f1 Ab8-calb2
Fish:	AB ucl4Tg (AB)
Anatomical Terms:	male organism cranial nerve II male organism retina male organism retinal cone cell male organism retinal ganglion cell retinal inner nuclear layer amacrine cell retinal inner nuclear layer retinal bipolar neuron
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ucl4Tg (AB)
Observed In:	male organism retina male organism retinal ganglion cell
Stage:	Adult

Phenotype Detail

Acknowledgments

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