Figure 4

Zebrafish LMNA Model Cardiac examination at 72 hours post fertilization (hpf). Modeling Qatar Genome Programme human LMNA cardiac variant produced specific cardiac phenotype in the zebrafish model, representative heart images at 72 hpf: (A) Zebrafish larvae at 72 hpf showing the two chambered heart (yellow square), and dorsal aorta flow rate (blue rectangle). Zebrafish examined groups were control, Morpholino injected group (MO lmna), human synthetic RNA of variant LMNA c.326 T > G (p.Val109Gly) (MO + LMNA^Variant) and wild-type (MO + LMNA^WT) co-injected with corresponding MO targeting the endogenous zebrafish transcript. Representative images of the heart chambers; atrium (a) and ventricle (v) (traced with yellow dotted line). (B) Heart rate for each individual fish was calculated as beats per minute (bpm) for the four experimental groups. Each dot represents an animal. Average heart rate of each group was compared to the control group. i: Control (n = 30), lmna MO (n = 17), MO + LMNA^Variant (n = 28), and MO + LMNA^WT (n = 13). ii: Control (n = 42), MO_0.35 (n = 32) and MO_0.5 (n = 15). ns: not significant.