FIGURE

Fig. 4

ID

ZDB-FIG-220623-40

Publication

Georgantzoglou et al., 2022 - A two-step search and run response to gradients shapes leukocyte navigation in vivo

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Fig. 4

Actin dynamics are required for gradient detection. (A and D) Neutrophils in a Tg(mpx:PHAKT-EGFP) before and after their response to laser wounding (LW; red dotted circle), in the presence (A) or absence (D) of LatB. Arrow points to cells showing local increases in PIP3 intensity. Scale bar = 25 μm. (B) Scheme that shows segmentation of immobilized neutrophils in two parts, facing the source or the opposite direction. (C) Average neutrophil PIP3 orientation (towards/away) in LatB-treated neutrophils, before and after response to gradient. n = 18 cells from 6 larvae. Two-tailed paired t test. (E) Scheme that shows the segmentation of neutrophils, with either unipolar or multipolar leading edge into two parts, one facing the source or the opposite direction. (F) Average neutrophil PIP3 orientation before and after response to gradient (towards/away). n = 9 cells from 7 larvae. Two-tailed paired t test, *, P = 0.0176. (C and F) Red dashed line denotes orientation equal to 1.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Biol.