ZFIN Figure: Wu et al., 2021, Fig. 4

ZFIN ID: ZDB-FIG-220621-38

ZFIN ID: ZDB-FIG-220621-38

Wu et al., 2021 - Reduced Vitellogenesis and Female Fertility in Gper Knockout Zebrafish. Frontiers in endocrinology 12:637691 Full text @ Front Endocrinol (Lausanne)

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	egfra esr1 esr2a esr2b
Antibody:	Ab1-egfra
Fish:	AB gper1^ecu18/ecu18 (AB)
Anatomical Terms:	brain liver ovarian follicle stage III ovary
Stage:	Adult

PHENOTYPE:

Fish:	gper1^ecu18/ecu18 (AB)
Observed In:	brain ovarian follicle stage III ovary ovary 17beta-estradiol ovary estrone
Stage:	Adult

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Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
egfra	AB	standard conditions	Adult	brain	RTPCR
			Adult	liver	RTPCR
			Adult	ovary	RTPCR
	gper1^ecu18/ecu18 (AB)	standard conditions	Adult	brain	RTPCR
			Adult	liver	RTPCR
			Adult	ovary	RTPCR
esr1	AB	standard conditions	Adult	brain	RTPCR
			Adult	liver	RTPCR
			Adult	ovary	RTPCR
	gper1^ecu18/ecu18 (AB)	standard conditions	Adult	brain	RTPCR
			Adult	liver	RTPCR
			Adult	ovary	RTPCR
esr2a	AB	standard conditions	Adult	ovary	RTPCR
	gper1^ecu18/ecu18 (AB)	standard conditions	Adult	ovary	RTPCR
esr2b	AB	standard conditions	Adult	brain	RTPCR
			Adult	liver	RTPCR
			Adult	ovary	RTPCR
	gper1^ecu18/ecu18 (AB)	standard conditions	Adult	brain	RTPCR
			Adult	liver	RTPCR
			Adult	ovary	RTPCR

Antibody Labeling Details

Antibody	Assay	Fish	Conditions	Stage	Anatomy
Ab1-egfra	WB	AB	standard conditions	Adult	ovarian follicle stage III
	WB	gper1^ecu18/ecu18 (AB)	standard conditions	Adult	ovarian follicle stage III

Phenotype Details

Fish	Conditions	Stage	Phenotype
gper1^ecu18/ecu18 (AB)	standard conditions	Adult	brain esr1 expression increased amount, abnormal
		Adult	brain esr2b expression increased amount, abnormal
		Adult	ovarian follicle stage III ab1-egfra labeling decreased amount, abnormal
		Adult	ovary 17beta-estradiol increased amount, abnormal
		Adult	ovary egfra expression decreased amount, abnormal
		Adult	ovary estrone increased amount, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)