FIGURE 5

The restoration of the full optokinetic response is accomplished between 10 and 14 days after light lesion dependent on the degree of difficulty. (A) Experimental timeline. The optokinetic response (OKR)-based vision test was performed before the light lesion (control) and at 3, 7, 10, 14, and 28 days post lesion (dpl). (B–J) Quantifications of the gain obtained for a black-white stimulus at a constant angular velocity of 15 dps but different combinations of contrast and spatial frequency revealed that the gain dropped significantly at 3 dpl and remains strikingly reduced at 7 dpl at all conditions. For easy conditions with high contrast and low spatial frequency (one red star), pre-lesion levels are reached at 10 dpl, whereas the OKR for medium difficulty (two red stars) returned to pre-lesion levels from 14 dpl onwards. Under hard conditions (three red stars) with low contrast and high spatial frequency, the gain was already very low at control recordings but statistically recovered by 10 dpl. All individual data points are summarized in Table 1. (K) Comparison of the relative tracking efficiency of all degrees of difficulty showed a significantly higher relative tracking efficiency at 7 and 10 dpl under easy conditions compared to medium and hard conditions. Statistics: all data are represented as mean ± SD, one-way ANOVA with Dunnett’s multiple comparison test against control (B–J), two-way ANOVA with Tukey’s multiple comparison test (K), p < 0.05 (*); 0.01 (**); 0.001 (***) or 0.0001 (****).